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KMID : 0043320100330010075
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Archives of Pharmacal Research
2010 Volume.33 No. 1 p.75 ~ p.80
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A New Norlupane Triterpene from the Leaves of Acanthopanax koreanum Increases the Differentiation of Osteoblastic MC3T3-E1 Cells
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Kim Young-Ho
Choi Eun-Mi
Kiem Phan Van
Park Sang-Hyouk
Nhiem Nguyen Xuan
Kim Jeong-Ah
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Abstract
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A new norlupane-triterpene, 3α,11α-dihydroxy-20,23-dioxo-30-norlupane-28-oic acid (1), and four known compounds; 3α,11α-dihydroxy-23-oxo-lup-20(29)-en-28-oic acid (2), 3α,11α-dihydroxylup-20(29)-en-28-oic acid (3), ent-kaur-16-en-19-oic acid (4), and betulabuside B (5) were isolated from the methanolic extract of the leaves of Acanthopanax koreanum. Their chemical structures were elucidated by mass, 1D- and 2D-NMR spectroscopy. To investigate the bioactivities of these compounds, their effects on the differentiation of osteoblastic MC3T3-E1 cells were tested. Compounds 3 and 4 significantly increased osteoblastic cell growth and differentiation as assessed by the MTT assay and collagen content, respectively (p < 0.05). Compounds 1 and 5 significantly increased the growth of MC3T3-E1 cells and caused a significant elevation of osteoblastic cell differentiation as assessed by the alkaline phosphatase activity. These results suggest that the compounds isolated from A.koreanum leaves have a direct stimulatory effect on bone formation in vitro and may contribute to the prevention for osteoporosis.
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KEYWORD
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Acanthopanax koreanum, Araliaceae, Lupane-triterpene, 3α, 11α-dihydroxy-20, 23 dioxo-30-norlupane-28-oic acid
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